本文關(guān)鍵給大家介紹了Python完成GB文件格式編碼序列編碼序列Fasta文件類型實(shí)例詳細(xì)說明，感興趣的小伙伴可以參考借鑒一下，希望可以有一定的幫助，祝愿大家盡可能發(fā)展，盡早工作上得到晉升

　　GB文件類型和FASTA文檔詳細(xì)介紹

　　在生物學(xué)中會(huì)有將GB文件格式編碼序列編碼序列成Fasta文件類型的需要，接下來我們運(yùn)用python腳本制作來解決這些問題。

　　gb格式文檔是GenBank的文檔，用于儲(chǔ)存編碼序列的詳細(xì)資料。包括一個(gè)gene的名字，序號(hào)，發(fā)現(xiàn)人，論文參考文獻(xiàn)，外顯子部位，編碼區(qū)編碼序列，蛋白序列等等信息。

　　比如：

　　LOCUS NM_213806 849 bp mRNA linear MAM 24-SEP-2019
　　DEFINITION Sus scrofa Fas ligand(TNF superfamily,member 6)(FASLG),mRNA.
　　ACCESSION NM_213806
　　VERSION NM_213806.1
　　KEYWORDS RefSeq.
　　SOURCE Sus scrofa(pig)
　　ORGANISM Sus scrofa
　　Eukaryota;Metazoa;Chordata;Craniata;Vertebrata;Euteleostomi;
　　Mammalia;Eutheria;Laurasiatheria;Cetartiodactyla;Suina;Suidae;
　　Sus.
　　REFERENCE 1(bases 1 to 849)
　　AUTHORS Lin F,Fu YH,Han J,Shen M,Du CW,Li R,Ma XS and Liu HL.
　　TITLE Changes in the expression of Fox O1 and death ligand genes during
　　follicular atresia in porcine ovary
　　JOURNAL Genet.Mol.Res.13(3),6638-6645(2014)
　　PUBMED 25177944
　　REMARK GeneRIF:Data suggest forkhead box protein O1(FoxO1)involvement
　　in the regulation of TNF-related apoptosis-inducing ligand TRAIL
　　and Fas ligand FasL expression during follicular atresia.
　　Publication Status:Online-Only
　　REFERENCE 2(bases 1 to 849)
　　AUTHORS Xie GH,Wang SJ,Wang Y,Zhang Y,Zhang HZ,Jin S,Wang QF,Liu ZC
　　and Ge HL.
　　TITLE Fas Ligand gene transfer enhances the survival of tissue-engineered
　　chondrocyte allografts in mini-pigs
　　JOURNAL Transpl.Immunol.19(2),145-151(2008)
　　PUBMED 18503890
　　REMARK GeneRIF:the result indicates that the expression of FasL by
　　chondrocytes is capable of inducing apoptosis of activated T cells
　　REFERENCE 3(bases 1 to 849)
　　AUTHORS Chang HW,Jeng CR,Lin CM,Liu JJ,Chang CC,Tsai YC,Chia MY and
　　Pang VF.
　　TITLE The involvement of Fas/FasL interaction in porcine circovirus type
　　2 and porcine reproductive and respiratory syndrome virus
　　co-inoculation-associated lymphocyte apoptosis in vitro
　　JOURNAL Vet.Microbiol.122(1-2),72-82(2007)
　　PUBMED 17321702
　　REMARK GeneRIF:The expression of FAS and FAS ligand in splenic
　　macrophages co-infected with porcine circovirus 2 and porcine
　　reproductive and respiratory syndrome virus is reported
　　REFERENCE 4(bases 1 to 849)
　　AUTHORS Tayade C,Black GP,Fang Y and Croy BA.
　　TITLE Differential gene expression in endometrium,endometrial
　　lymphocytes,and trophoblasts during successful and abortive embryo
　　implantation
　　JOURNAL J.Immunol.176(1),148-156(2006)
　　PUBMED 16365405
　　REFERENCE 5(bases 1 to 849)
　　AUTHORS Bai L,Maedler K,Donath M and Tuch BE.
　　TITLE Expression of Fas but not Fas ligand on fetal pig beta cells
　　JOURNAL Xenotransplantation 11(5),426-435(2004)
　　PUBMED 15303979
　　REMARK GeneRIF:FasL was not detected on fetal pig pancreatic cells but
　　could be induced on both beta and non-beta cells when the cells
　　were treated with IL1beta.
　　Erratum:[Xenotransplantation.2016 Mar;23(2):171-2.PMID:27106874]
　　REFERENCE 6(bases 1 to 849)
　　AUTHORS Tsuyuki S,Kono M and Bloom ET.
　　TITLE Cloning and potential utility of porcine Fas ligand:overexpression
　　in porcine endothelial cells protects them from attack by human
　　cytolytic cells
　　JOURNAL Xenotransplantation 9(6),410-421(2002)
　　PUBMED 12371937
　　REFERENCE 7(bases 1 to 849)
　　AUTHORS Motegi-Ishiyama Y,Nakajima Y,Hoka S and Takagaki Y.
　　TITLE Porcine Fas-ligand gene:genomic sequence analysis and comparison
　　with human gene
　　JOURNAL Mol.Immunol.38(8),581-586(2002)
　　PUBMED 11792426
　　REFERENCE 8(bases 1 to 849)
　　AUTHORS Muneta Y,Shimoji Y,Inumaru S and Mori Y.
　　TITLE Molecular cloning,characterization,and expression of porcine Fas
　　ligand(CD95 ligand)
　　JOURNAL J.Interferon Cytokine Res.21(5),305-312(2001)
　　PUBMED 11429161
　　COMMENT PROVISIONAL REFSEQ:This record has not yet been subject to final
　　NCBI review.The reference sequence was derived from AB027297.1.
　　##Evidence-Data-START##
　　Transcript exon combination::AB027297.1,AF397407.1[ECO:0000332]
　　RNAseq introns::single sample supports all introns
　　SAMN01893940,SAMN01915393
　　[ECO:0000348]
　　##Evidence-Data-END##
　　FEATURES Location/Qualifiers
　　source 1..849
　　/organism="Sus scrofa"
　　/mol_type="mRNA"
　　/db_xref="taxon:9823"
　　/chromosome="9"
　　/map="9"
　　gene 1..849
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/note="Fas ligand(TNF superfamily,member 6)"
　　/db_xref="GeneID:396726"
　　CDS 1..849
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/note="CD95 ligand;tumor necrosis factor(ligand)
　　superfamily,member 6;fas antigen ligand"
　　/codon_start=1
　　/product="tumor necrosis factor ligand superfamily member
　　6"
　　/protein_id="NP_998971.1"
　　/db_xref="GeneID:396726"
　　/translation="MQQPFNYPYPQIFWVDSSATSPWASPGSVFPCPASVPGRPGQRR
　　PPPPPPPPPPPPTLLPSRPLPPLPPPSLKKKRDHNAGLCLLVMFFMVLVALVGLGLGM
　　FQLFHLQKELTELRESASQRHTESSLEKQIGHPNLPSEKKELRKVAHLTGKPNSRSIP
　　LEWEDTYGIALVSGVKYMKGSLVINDTGLYFVYSKVYFRGQYCNNQPLSHKVYTRNSR
　　YPQDLVLMEGKMMNYCTTGQMWARSSYLGAVFNLTSADHLYVNVSELSLVNFEESKTF
　　FGLYKL"
　　mat_peptide 1..390
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/product="ADAM10-processed FasL form.{ECO:0000250}"
　　/experiment="experimental evidence,no additional details
　　recorded"
　　/note="propagated from UniProtKB/Swiss-Prot(Q9BEA8.1)"
　　mat_peptide 1..249
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/product="FasL intracellular domain.{ECO:0000250}"
　　/experiment="experimental evidence,no additional details
　　recorded"
　　/note="propagated from UniProtKB/Swiss-Prot(Q9BEA8.1)"
　　misc_feature 244..249
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/experiment="experimental evidence,no additional details
　　recorded"
　　/note="Cleavage,by SPPL2A.{ECO:0000250};propagated from
　　UniProtKB/Swiss-Prot(Q9BEA8.1);cleavage site"
　　misc_feature 247..309
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/experiment="experimental evidence,no additional details
　　recorded"
　　/note="propagated from UniProtKB/Swiss-Prot(Q9BEA8.1);
　　transmembrane region"
　　misc_feature 388..393
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/experiment="experimental evidence,no additional details
　　recorded"
　　/note="Cleavage,by ADAM10.{ECO:0000250};propagated from
　　UniProtKB/Swiss-Prot(Q9BEA8.1);cleavage site"
　　mat_peptide 391..846
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/product="Tumor necrosis factor ligand superfamily member
　　6,soluble form.{ECO:0000250}"
　　/experiment="experimental evidence,no additional details
　　recorded"
　　/note="propagated from UniProtKB/Swiss-Prot(Q9BEA8.1)"
　　misc_feature 553..555
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/experiment="experimental evidence,no additional details
　　recorded"
　　/note="N-linked(GlcNAc...)asparagine.{ECO:0000255};
　　propagated from UniProtKB/Swiss-Prot(Q9BEA8.1);
　　glycosylation site"
　　misc_feature 751..753
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/experiment="experimental evidence,no additional details
　　recorded"
　　/note="N-linked(GlcNAc...)asparagine.{ECO:0000255};
　　propagated from UniProtKB/Swiss-Prot(Q9BEA8.1);
　　glycosylation site"
　　misc_feature 781..783
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/experiment="experimental evidence,no additional details
　　recorded"
　　/note="N-linked(GlcNAc...)asparagine.{ECO:0000255};
　　propagated from UniProtKB/Swiss-Prot(Q9BEA8.1);
　　glycosylation site"
　　exon 1..351
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/inference="alignment:Splign:2.1.0"
　　exon 352..397
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/inference="alignment:Splign:2.1.0"
　　exon 398..454
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/inference="alignment:Splign:2.1.0"
　　exon 455..849
　　/gene="FASLG"
　　/gene_synonym="CD95-L;FASL;TNFSF6"
　　/inference="alignment:Splign:2.1.0"

　　ORIGIN

　　1 atgcagcagc ccttcaatta cccatacccc caaatcttct gggtggacag cagtgctacc

　　61 tctccctggg cctccccagg ctcagtcttc ccctgtccag cttctgtgcc aggaaggcca

　　121 gggcaaagga ggccaccacc accaccgccg ccaccgccac caccaccaac actcctgcca

　　181 tcaagaccgc tgcctccact gccaccgcca tctctgaaga agaagaggga ccacaatgca

　　241 ggcctgtgtc tccttgtgat gttcttcatg gttctggtgg ccctggttgg attggggctg

　　301 gggatgtttc agctcttcca cctacagaag gagctgactg aactcagaga gtctgccagc

　　361 caaaggcata cagaatcatc tttggagaag caaataggtc accccaatct accctctgag

　　421 aaaaaggagc tgagaaaagt ggcccactta acaggcaagc ctaactcaag atccatccct

　　481 ctggaatggg aagacaccta tggaattgcc ttggtctctg gggtgaagta tatgaagggc

　　541 agccttgtga tcaatgacac tgggctgtat tttgtgtatt ccaaagtgta cttccggggt

　　601 cagtactgca acaaccagcc cctgagtcac aaggtataca caaggaactc taggtatccc

　　661 caggacctgg tgctgatgga gggaaagatg atgaactatt gcactactgg ccaaatgtgg

　　721 gcccgcagca gctacctggg ggctgtgttc aatctcacca gcgctgacca tttatatgtc

　　781 aacgtatctg agctctctct ggtcaatttt gaggaatcta agacattttt tggcttatat

　　841 aagctctga

　　//

　　fasta格式是一種基于文本用于表示核酸序列或多肽序列的格式。其中核酸或氨基酸均以單個(gè)字母來表示，且允許在序列前添加序列名及注釋。該格式已成為生物信息學(xué)領(lǐng)域的一項(xiàng)標(biāo)準(zhǔn)。

　　例如：

　　>NM_213806

　　ATGCAGCAGCCCTTCAATTACCCATACCCCCAAATCTTCTGGGTGGACAGCAGTGCTACC

　　TCTCCCTGGGCCTCCCCAGGCTCAGTCTTCCCCTGTCCAGCTTCTGTGCCAGGAAGGCCA

　　GGGCAAAGGAGGCCACCACCACCACCGCCGCCACCGCCACCACCACCAACACTCCTGCCA

　　TCAAGACCGCTGCCTCCACTGCCACCGCCATCTCTGAAGAAGAAGAGGGACCACAATGCA

　　GGCCTGTGTCTCCTTGTGATGTTCTTCATGGTTCTGGTGGCCCTGGTTGGATTGGGGCTG

　　GGGATGTTTCAGCTCTTCCACCTACAGAAGGAGCTGACTGAACTCAGAGAGTCTGCCAGC

　　CAAAGGCATACAGAATCATCTTTGGAGAAGCAAATAGGTCACCCCAATCTACCCTCTGAG

　　AAAAAGGAGCTGAGAAAAGTGGCCCACTTAACAGGCAAGCCTAACTCAAGATCCATCCCT

　　CTGGAATGGGAAGACACCTATGGAATTGCCTTGGTCTCTGGGGTGAAGTATATGAAGGGC

　　AGCCTTGTGATCAATGACACTGGGCTGTATTTTGTGTATTCCAAAGTGTACTTCCGGGGT

　　CAGTACTGCAACAACCAGCCCCTGAGTCACAAGGTATACACAAGGAACTCTAGGTATCCC

　　CAGGACCTGGTGCTGATGGAGGGAAAGATGATGAACTATTGCACTACTGGCCAAATGTGG

　　GCCCGCAGCAGCTACCTGGGGGCTGTGTTCAATCTCACCAGCGCTGACCATTTATATGTC

　　AACGTATCTGAGCTCTCTCTGGTCAATTTTGAGGAATCTAAGACATTTTTTGGCTTATAT

　　AAGCTCTGA

　　處理步驟

　　將文件夾下gb文件批量讀取

　　將各個(gè)gb文件中的登錄號(hào)和具體序列抽提出來，并寫入fasta文件

　　將fasta文件進(jìn)一步處理去掉換行符，使一個(gè)完整的序列中沒有換行符

　　將所有處理好的fasta文件存入一個(gè)新建的子文件夾中

　　Python腳本代碼如下：

　　#!/usr/bin/env python
　　#-*-encoding:utf-8-*-
　　'''
　　File:gb2fasta.py
　　Time:2020/07/04 14:15:13
　　Author:Ai
　　Version:1.0
　　Contact:aqy0716 163.com
　　License:(C)Copyright 2020 SCAU
　　Desc:將gb文件轉(zhuǎn)換為fasta文件，同時(shí)轉(zhuǎn)成無換行符，最后存入新的子文件夾中
　　'''
　　#here put the import lib
　　import os
　　import shutil
　　def gb2fasta(path):
　　#從gb文件中抽取登錄號(hào)和具體序列信息存入同名fasta文件
　　#讀取文件夾相關(guān)信息
　　for root,dirs,files in os.walk(path):
　　for file in files:
　　#打印文件所屬目錄
　　print(root+''+file)
　　#獲取文件路徑
　　path_gb=os.path.join(root,file)
　　flag=0
　　if path_gb[-2:]=='gb':
　　#打開新建fasta文件準(zhǔn)備寫入
　　fasta=open(path_gb[:-2]+'fasta','w')
　　#打開gb文件，準(zhǔn)備讀取序列信息并寫入fasta文件
　　with open(path_gb,'r')as f:
　　#逐行掃描
　　for line in f:
　　#如果是ACCESSION行，則寫入fasta文件作為序列標(biāo)題
　　if line[0:9]=='ACCESSION':
　　fasta.writelines('>'+line.split()[1]+'n')
　　#如果是ORGIN行，代表是序列
　　elif line[0:6]=='ORIGIN':
　　flag=1
　　elif flag==1:
　　#通過空格符（空格換行制表）對(duì)字符串進(jìn)行切片
　　s=line.split()
　　#非空切片字符打印
　　if s!=[]:
　　#print(s)
　　#去掉列表首個(gè)元素（數(shù)字序號(hào)）后，連接所有元素即為完整序列按行寫入fasta文件
　　seq=''.join(s[1:])
　　fasta.writelines(seq.upper()+'n')
　　fasta.close()
　　def multi2single(path):
　　#此函數(shù)功能為：將多行序列轉(zhuǎn)換為單行序列（即去掉換行符），成為標(biāo)準(zhǔn)fasta文件
　　for root,dirs,files in os.walk(path):
　　for file in files:
　　path_full=os.path.join(root,file)
　　#有fasta且不為single.fasta，才進(jìn)行單行轉(zhuǎn)換，否則會(huì)重復(fù)創(chuàng)建文件夾
　　a=path_full[-5:]=='fasta'
　　if path_full[-12:]=='single.fasta':
　　b=True
　　else:
　　b=False
　　b=bool(1-b)
　　if a&b:
　　fr=open(path_full,'r')
　　fw=open(path_full[:-6]+'_single.fasta','w')
　　seq={}
　　for line in fr:
　　if line.startswith('>'):#判斷字符串是否以‘>開始'
　　name=line.split()[0]#以空格為分隔符，并取序列為0的項(xiàng)。
　　seq[name]=''
　　else:
　　seq[name]+=line.replace('n','')
　　fr.close()
　　for i in seq.keys():
　　fw.write(i)
　　fw.write('n')
　　fw.write(seq<i>)
　　fw.write('n')
　　fw.close()
　　def copy2subdir(path):
　　#將生成的_single.fasta文件存入新的子文件夾.singl_fasta中
　　root1=path
　　for root,dirs,files in os.walk(path):
　　subdir=os.path.join(root1,'singl_fasta')
　　for file in files:
　　oldfile=os.path.join(root1,str(file))
　　newfile=os.path.join(root1,'singl_fasta',str(file))
　　if not os.path.exists(subdir):
　　os.makedirs(subdir)
　　print("目錄創(chuàng)建成功！")
　　if oldfile[-12:]=='single.fasta':
　　if not os.path.exists(newfile):
　　shutil.copyfile(oldfile,newfile)
　　print("n你的fasta文件保存在:"+subdir+"文件夾下n")
　　if __name__=="__main__":
　　path=input("請(qǐng)輸入路徑")#此處輸入D:docugb2fasta
　　gb2fasta(path)
　　multi2single(path)
　　copy2subdir(path)

　　運(yùn)行情況

　　PS D:vscode_python_magic>&d:/ruanjiancangku/python_projectkotin/venv/Scripts/python.exe d:/vscode_python_magic/實(shí)驗(yàn)室-magic/序列處理/gb2fasta.py
　　D:docugb2fasta1-FASLG-swine-849bp-NM_213806.fasta
　　D:docugb2fasta1-FASLG-swine-849bp-NM_213806.gb
　　['1','atgcagcagc','ccttcaatta','cccatacccc','caaatcttct','gggtggacag','cagtgctacc']
　　['61','tctccctggg','cctccccagg','ctcagtcttc','ccctgtccag','cttctgtgcc','aggaaggcca']
　　['121','gggcaaagga','ggccaccacc','accaccgccg','ccaccgccac','caccaccaac','actcctgcca']
　　['181','tcaagaccgc','tgcctccact','gccaccgcca','tctctgaaga','agaagaggga','ccacaatgca']
　　['241','ggcctgtgtc','tccttgtgat','gttcttcatg','gttctggtgg','ccctggttgg','attggggctg']
　　['301','gggatgtttc','agctcttcca','cctacagaag','gagctgactg','aactcagaga','gtctgccagc']
　　['361','caaaggcata','cagaatcatc','tttggagaag','caaataggtc','accccaatct','accctctgag']
　　['421','aaaaaggagc','tgagaaaagt','ggcccactta','acaggcaagc','ctaactcaag','atccatccct']
　　['481','ctggaatggg','aagacaccta','tggaattgcc','ttggtctctg','gggtgaagta','tatgaagggc']
　　['541','agccttgtga','tcaatgacac','tgggctgtat','tttgtgtatt','ccaaagtgta','cttccggggt']
　　['601','cagtactgca','acaaccagcc','cctgagtcac','aaggtataca','caaggaactc','taggtatccc']
　　['661','caggacctgg','tgctgatgga','gggaaagatg','atgaactatt','gcactactgg','ccaaatgtgg']
　　['721','gcccgcagca','gctacctggg','ggctgtgttc','aatctcacca','gcgctgacca','tttatatgtc']
　　['781','aacgtatctg','agctctctct','ggtcaatttt','gaggaatcta','agacattttt','tggcttatat']
　　['841','aagctctga']
　　['//']
　　D:docugb2fasta2-LTA-swine-1584bp-NM_214453.fasta
　　D:docugb2fasta2-LTA-swine-1584bp-NM_214453.gb
　　['1','agaaaggggc','ccacaggggt','cccgcacagc','aggtgagact','ctcccacccc','atctcctagg']
　　['61','gctgtccggg','tgctggactc','ccccctcact','tcggtccctc','cgcccgctcc','ctggccttcc']
　　['121','tgcccctcct','gcatcttcac','cccggcctgg','gccttggtgg','gtttggtttt','ggtttgttct']
　　['181','ctctgattct','ttatctgtca','ggctctttct','agctctcaca','cactctgatc','cctctctgtt']
　　['241','cccttcccat','ctctgtttct','ctctgggtct','ccccctgctc','acctcgggat','ttccctgagt']
　　['301','gcctctggtc','cccttctctg','tctggcgccc','cgtctcttgt','ctctcggggt','ggctgtctcc']
　　['361','gagggcagga','ggccttcttc','cgcaggtgcc','ccgccccgct','cactgtctct','ctccccccac']
　　['421','aggttttccc','catgacacca','cctggacgcc','tctacctccg','gagggtgtgc','agcaccccca']
　　['481','tcctcctcct','cctggggctg','ctgctggccc','tgccgcccga','ggcccagggg','ctccctggcg']
　　['541','tcggcctccc','accctcagct','gcacagcctg','cccatcagca','ccccccaaag','cacttggcca']
　　['601','gaggcaccct','caaacctgcc','gctcacctcg','ttggagaccc','cagcaccccg','gactcactgc']
　　['661','gctggagagc','gaacacggat','cgtgccttcc','tccgccatgg','cttcttgctg','agcaacaact']
　　['721','ccctgctggt','ccccaccagt','ggcctctact','ttgtctactc','ccaggtcgtc','ttctccgggg']
　　['781','aaggctgctt','ccccaaggcc','acccccaccc','ctctctacct','ggcccacgag','gtccagctct']
　　['841','tctcctccca','gtaccccttc','cacgtgccgc','tcctcagcgc','tcagaagtcc','gtgtgccccg']
　　['901','ggccacaggg','accttgggtg','cgctctgtgt','accagggggc','tgtgttcctg','ctcacccagg']
　　['961','gagatcagct','gtccacacac','acagacggca','ccccccacct','gctcctcagc','cccagtagcg']
　　['1021','tcttctttgg','agccttcgct','ctatagaaga','atccagaaag','aaaaaaattg','gtttcaaggc']
　　['1081','cttctcccct','tttcacctcc','cttatgacca','cttcggaggt','caccgcgcct','ctcctctgac']
　　['1141','aatttccaac','agtctcatct','tcccccacgc','tcagcacctg','gagcttctgt','agaaggaatt']
　　['1201','ctaggcacct','cgggggaact','ggaaccaccc','cggatgctct','gctgaggatc','tgaatgcccg']
　　['1261','cctggagccc','ttcccctgtc','ctgcccgtct','aggggccctc','gtccaggacg','tggaagggaa']
　　['1321','gctgacccat','gagggacttt','gaacggatga','ccggagcggt','gtgggggggt','tatttatgaa']
　　['1381','ggggaaaatt','aaattattta','tttatggagg','atggagagaa','gggaatcaca','gagggatgtc']
　　['1441','agaagagtgt','gacacatgtg','cccaagagat','aaagtgacag','aaggcatggg','ctccagatga']
　　['1501','cccggccaga','gagggcaaag','tggctcagga','aggggctgct','tgactggagg','ctcatgagga']
　　['1561','gacggctgac','cctcgatgaa','accc']
　　['//']
　　D:docugb2fasta3-LTB-swine-950bp-NM_001185138.fasta
　　D:docugb2fasta3-LTB-swine-950bp-NM_001185138.gb
　　['1','tcggatgggg','gcaccggggc','tggagggccg','gggtaggagg','ccccagggga','agggatgcct']
　　['61','cctgctggcc','gtggcagggg','ccacttccct','ggtgaccctc','ctgctggccg','tgcctatcac']
　　['121','ggtcctggct','gtgctggcct','tggtgcccca','ggagcaggga','gaactggtaa','cagggaccgc']
　　['181','tgacccaggc','acccaggcgg','aggcccagca','gcgattggag','tccaaggaga','cgccagagga']
　　['241','ggaggcagaa','acagatctca','gccccaggct','cccagctgcc','cacctcattg','gcgcttggat']
　　['301','cacgggtcag','gggctaggct','gggaggcgaa','gaaagaagag','gcgtttctga','ggagcgggac']
　　['361','gcagttctct','ggcgcggagg','gcctggccct','cccgcaggac','ggcctctact','acctctactg']
　　['421','tcacgtcggc','taccggggcc','gggcacctcc','tcccggcggg','gaccccctgg','accgctcggt']
　　['481','cacgctgctc','agccggctgt','accgggcggg','gggcgcctac','ggaccgggga','ctcccgagct']
　　['541','gctgctggag','ggcgcggaga','ctgtgactcc','ggtcttggac','cccagtcgga','ggcacgagta']
　　['601','cgggcccctc','tggtacacga','gcgtggggtt','cggtggcctg','gtgcagctcc','ggaggggcga']
　　['661','gagggtgtac','gttaatatca','gtcaccccga','tatggtggat','tacaggagag','gaaagacctt']
　　['721','cttcggggcg','gtgatggtgg','gctgaggact','gtccgcggcc','cgagaggacc','actgcatggt']
　　['781','gggagtgtgt','cgatggatca','agcccagaca','cggggtccca','gacaccaggc','cagacaccat']
　　['841','ggccgtgggg','aaaatgcagg','agatcgtgtg','gaaaactgat','tttgagcctg','atgaaaataa']
　　['901','agaatgtaaa','agctttaatn','gctgcccatg','ccaaaaaaaa','aaaaaaaaaa']
　　['//']
　　D:docugb2fasta4-TNF-swine-1666bp-NM_214022.fasta
　　D:docugb2fasta4-TNF-swine-1666bp-NM_214022.gb
　　['1','cccagagtga','ggacaccagg','ggaccagcca','ggagagagac','aagccatctc','caggaccccc']
　　['61','tagaaataac','ctctcagaag','acacaccccc','gaacaggcag','ccggacgact','ctctccctct']
　　['121','cacacgctgc','cccggggcgc','caccatctcc','cagctggacc','tgagcccctc','tgaaaaagac']
　　['181','accatgagca','ctgagagcat','gatccgagac','gtggagctgg','cggaggaggc','gctcgccaag']
　　['241','aaggccgggg','gcccccaggg','ctccaggagg','tgcctgtgcc','tcagcctctt','ctccttcctc']
　　['301','ctggtcgcag','gagccaccac','gctcttctgc','ctactgcact','tcgaggttat','cggcccccag']
　　['361','aaggaagagt','ttccagctgg','ccccttgagc','atcaaccctc','tggcccaagg','actcagatca']
　　['421','tcgtctcaaa','cctcagataa','gcccgtcgcc','cacgttgtag','ccaatgtcaa','agccgaggga']
　　['481','cagctccaat','ggcagagtgg','gtatgccaat','gccctcctgg','ccaacggcgt','gaagctgaaa']
　　['541','gacaaccagc','tggtggtgcc','gacagatggg','ctgtacctca','tctactccca','ggtcctcttc']
　　['601','aggggccaag','gctgcccttc','caccaacgtt','ttcctcactc','acaccatcag','ccgcatcgcc']
　　['661','gtctcctacc','agaccaaggt','caacctcctc','tctgccatca','agagcccttg','ccagagggag']
　　['721','acccccgagg','gggccgaggc','caagccctgg','tacgaaccca','tctacctggg','aggggtcttc']
　　['781','cagctggaga','aggatgatcg','actcagtgcc','gagatcaacc','tgcccgacta','tctggacttt']
　　['841','gctgaatctg','ggcaggtcta','ttttgggatc','attgccctgt','gagggggcag','gacatccgtt']
　　['901','ccctcccctg','tccatccctt','tattatttta','ctccttcaga','ccccctcacg','tccttctggt']
　　['961','ttagaaagag','aatgaggggc','tggggactgg','gctccaagct','taaaacttta','aacaacaaca']
　　['1021','gcaacactta','gaaatcaggg','attcagggat','gtgtggcctg','gacaaccagg','cactgaccac']
　　['1081','caccaagaat','tggaactggg','gcttccagac','tcgctggggt','ccttgggttt','ggattcctgg']
　　['1141','atgcaacctg','ggacatctgg','aatgtggctg','ccagggaagc','ttgggttcca','atcggaatac']
　　['1201','ttcagaacat','tccttgagaa','gatttcacct','caatcttgat','gactttttag','gcttcccttt']
　　['1261','cttccaattt','tccagacttc','cctgggatgg','ggagcccagc','cccaaacccc','acaggccagc']
　　['1321','tccctcttat','ttatatttgc','acttggcatt','attatttatt','tatttattta','ttatttattt']
　　['1381','actagtgaat','gtatttattc','aggagggcga','ggtgtcctgg','gagacccagc','ataagggctg']
　　['1441','ccttggttca','gatgtgtttt','ctgtgaaaac','ggagctgaac','tgtaggttgc','tcccacctgg']
　　['1501','cctcctagcc','tctgtgcctc','cttttgctta','tgtttttaaa','aacaaatatt','tatctgatcg']
　　['1561','agttgtctaa','ataatgctga','tttggtgact','aacttgtcgc','tacatcgctg','aacctctgct']
　　['1621','ccccagggga','gttgtgtctg','taaccgccct','actggtcagt','ggcgag']
　　['//']
　　D:docugb2fasta5-TNFSF4-swine-549bp-NM_001025217.fasta
　　D:docugb2fasta5-TNFSF4-swine-549bp-NM_001025217.gb
　　['1','atggaagggg','tccaacccct','agatgaaaat','gtgggaaacg','caccaggacg','aagactcttg']
　　['61','aggaacaagc','tattgttggt','ggcctccgta','attcagggtc','tggggttgct','cctgtgtctc']
　　['121','acctacatct','gcctgcacct','ctatgctcag','gtgccatctc','agtaccctcc','aattcagagt']
　　['181','atcaaagtac','aatttaccaa','gtgtgaaaat','gataatggtt','tcatcatcac','accctcaagc']
　　['241','aaggatggaa','ccatgaaagt','gcaaaacaac','tcaatcatca','tcaactgtga','tgggttctat']
　　['301','ctcatctccc','tgaagggtta','cttttctcag','gagctcagcc','tcatgcttca','gtaccggaag']
　　['361','ggtcggaaac','ctctcttctc','cctgaacaag','gtcaagtctg','tggactctgt','cacagtagcc']
　　['421','gatctggctt','tcaaggacaa','ggtcttcctg','aacgtgacca','ctcatagtgc','ctcctgtgaa']
　　['481','gacattcagg','tgaatggtgg','ggaattgatt','ctcattcatc','aaaatcctgg','tggattctgt']
　　['541','gtctactga']
　　['//']
　　D:docugb2fasta6-TNFSF10-swine-1696bp-NM_001024696.fasta
　　D:docugb2fasta6-TNFSF10-swine-1696bp-NM_001024696.gb
　　['1','agcagtcaga','ccctgcctgg','accatggcgg','tgatgcagac','tccaggaggc','cccagccccg']
　　['61','ggcagacctg','tgtgttgatc','ctgatcttca','cagtgctcct','gcaagccctc','tgtgtggcct']
　　['121','tgacttacgt','gtacttcacc','aatgaactga','aacagatgca','ggacaagtac','tccaaaagcg']
　　['181','gtatagcttg','cttcttaaag','gaagatgaca','gtttctggga','tcccaccgat','gacgagagaa']
　　['241','tgctcagccc','ctgctggcag','gtgaagtggc','agctacgtca','gtttgtgaga','aagatgattt']
　　['301','tgagaaccta','tgaggaaacc','atttctacag','tttcagaaaa','gcaacaaggc','attcctcacc']
　　['361','tagaaagaga','aaaaggtcca','cagagagtgg','ctgctcacat','aactggaacc','agtaggaaaa']
　　['421','gaagcacatt','tccatctcta','agctccaaat','atgaaaaagc','tttgggccag','aaaataaact']
　　['481','cctgggaatc','atcaagaaaa','ggacattcat','tcttgaataa','ttttcacttg','aggaatggag']
　　['541','agctggttat','ccatcaaaca','gggttttact','acatctattc','ccaaacatac','tttcgatttc']
　　['601','aggaacctga','ggaaattttg','ggaacggttt','ctacagaagg','gaacagaaag','aaaaacaggc']
　　['661','aaatgataca','gtatatttac','aaatggacaa','gctatcctga','ccctatactg','ctgatgaaaa']
　　['721','gtgctagaaa','tagttgttgg','tctaaagatt','cagaatatgg','actctattcc','atctatcaag']
　　['781','gtggaatatt','tgagcttaag','gaagatgacc','gaatttttgt','ctctgttact','aatgagcaac']
　　['841','tgattgacat','ggaccaagaa','gccagttttt','tcggggcctt','tttaattggc','taaatgatct']
　　['901','gcagggaaaa','aaaccatgcc','ccagagtgac','tattcagagt','cgtatactgt','gaaaatattc']
　　['961','cagcagagcc','aataggttaa','ggcagcctga','gcaaagaggc','ctcaacccaa','aggctcaaca']
　　['1021','acacaagctt','tttggaaagt','gaaaagtgac','caattccttc','caggaaaatg','aaactgccaa']
　　['1081','gagaccttgt','ggagctctgc','ctgatgtcat','tttgctagta','aacatctaga','agatactctg']
　　['1141','tctccaaatt','tgtgtaacaa','ttaacacctc','ctgcctttat','catctaatcc','tgtgaagatt']
　　['1201','ctagaagaaa','gagtagtgat','ccatctcagg','tgggaataag','ggacaacatt','cccaaaacta']
　　['1261','aagagaaaag','ggcagcactg','aaaggtcaca','gtcaatatat','gcagtttcag','tacaaacata']
　　['1321','acaaattaaa','gctacgttta','gtggacaagg','agctacttct','gaatggtttg','tgcttttctc']
　　['1381','tactaaaaat','caggctggcc','aaaagcactc','agggtatttt','tgataaagga','ctctaaaata']
　　['1441','agtgataaag','tatggcgata','cctcagaaaa','ctaaatacag','aactaccaca','tgacccagca']
　　['1501','atcccactcc','tgtgcatata','tctggacaaa','actttccttg','aaaaagatac','attcatctct']
　　['1561','atgctcattg','cagcactatt','cacagtagcc','aagacatgga','aacacctata','tgtctatgaa']
　　['1621','tggatgaata','gattaagaag','gtgtgttatg','tatacatant','ggaatactgg','gaagccataa']
　　['1681','aaaggacaaa','gaggcc']
　　['//']
　　D:docugb2fasta7-TNFSF13b-swine-1013bp-NM_001097498.fasta
　　D:docugb2fasta7-TNFSF13b-swine-1013bp-NM_001097498.gb
　　['1','tctaggaggg','aaatggatga','ctccacgggg','gagcagtcac','gcctttcttg','ccttagcacg']
　　['61','agagaagaaa','tgaaactgaa','ggagacggtc','cccatcctcc','cccagaagga','aagcccctct']
　　['121','gtccgcatct','ccaaagatgg','gaagctgctg','gtcgtgaccc','tgctgctggc','cctgctgtcc']
　　['181','tgctgcctca','cggggatctt','tgcaccacca','gctccaaggg','agagcagctc','cattcaaagc']
　　['241','aacaggagta','agcgcgccgc','gcaggatgcg','gaggagacag','tcactcagga','ctgcttgcaa']
　　['301','ttgattgcag','acagtgacat','gcctactata','cgaaaaggag','cttatacatt','tgttccatgg']
　　['361','cttctcagct','ttaaaagagg','aagagcccta','gaagaaaaag','aaaataaaat','cgtggtcaaa']
　　['421','gaaacgggtt','acttttttat','atacggtcag','gttttataca','ccgataacac','ctttgccatg']
　　['481','gggcatctca','tacagaggaa','gaaagtccat','gtctttgggg','atgaactgag','tctggtgact']
　　['541','ttgttccgat','gtattcaaaa','tatgcctgaa','acactaccca','ataattcctg','ttattcagct']
　　['601','ggcattgcaa','agctggagga','aggagatgaa','ctccaactgg','caataccacg','tgaagacgct']
　　['661','aaaatatcac','gggatggaga','cggcacattt','tttggtgcat','tgaaacttct','gtgacctact']
　　['721','tacaccttgt','ttgtggctct','tgccctccct','ccctctgtac','ctctaaagag','aaaacactta']
　　['781','actggaaata','ccaaaagggg','aaaaaaaagt','agttaccata','gccttttctg','tgagctgttt']
　　['841','gttttggttt','gctgaaacta','gaccaaaaca','ggaaatttaa','cagacaacca','cagccaaagg']
　　['901','gtatcatgtg','aattacaaga','aatagagccc','atttaagaaa','aaatagaatt','agaaagactt']
　　['961','ttcactgtaa','tgccatgttg','aacagcttag','tcatagcttc','ttgtcttgga','gga']
　　['//']
　　D:docugb2fasta8-TNFSF18-swine-5005bp-XM_005667782.fasta
　　D:docugb2fasta8-TNFSF18-swine-5005bp-XM_005667782.gb
　　['1','gcattccatt','taacaaatga','aaaggctaag','gcataaagaa','ccaggagaga','gaaccggaga']
　　['61','tttcctcaat','tttagtgtag','taaatcaaca','gttttagtgc','taggaagttt','tttggaatag']
　　['121','agtgtaaact','cagatggtag','gacagggtgc','atgaaaatat','ccttttctat','gataacttta']
　　['181','tttctgtctg','atgtcagcat','tgaaatttca','gagtattaaa','atggtgaggt','atgaaaaaac']
　　['241','taagcttgtt','gttattgaca','tatttttaaa','aataaaattc','tagtaataca','ttactgtttt']
　　['301','ctagagatta','tctcagaatg','gacacattgt','ataccctagc','aattgatgaa','aatatttttc']
　　['361','ccaaaccatg','aaccccagca','tccttagctc','cctgacctac','tgcctcccac','aaacatgata']
　　['421','tttggagtat','gatagacctt','catcttgaat','ttcattcttt','ttcttacaaa','agtaattttc']
　　['481','ttatctggaa','ataatttgta','atgttgaata','gttccatagt','tcctcttgct','tcagaaaata']
　　['541','tttatttttc','ctcttaactt','cccttgttgg','gttttttttt','tttaaatcat','ctgtgtttgt']
　　['601','gttggcttct','agccctcagt','tccagcacct','ttggtctggt','gccaaatgtt','agtcagcact']
　　['661','taggctaaaa','gtatcgtttt','ccaacaccca','gatcagaagg','aaaactccgc','ctcttacacc']
　　['721','cactacttag','tgctatacta','caaaactgac','tagttgaatc','atgtgctcat','tacttctgaa']
　　['781','tttctgcttt','tcacaactct','cattcctgca','gagaatgagt','ttgagccaca','tggagaatat']
　　['841','gcctttaagc','cattcaagtc','ctcacgcagc','acagagacca','tcctggaagc','aatggctact']
　　['901','ctactcaaca','atagttattt','tgctattact','ttgctccttc','agtgcactaa','tcttaacttt']
　　['961','tctcccactc','aagacctcca','acaggccatg','tgtagcgaag','tttggaccat','taccttcaaa']
　　['1021','atggcaaatg','ccatctcctg','agccttcttg','tgtgaataag','acagatgatt','ggaggctgaa']
　　['1081','gatacttcag','aatggcttgt','atttaattta','tggccaagtg','gctcccaaca','cagcttacaa']
　　['1141','ggggcaagct','ccttttgagg','tgttgctacg','taggaatgaa','gaccccatac','aatctctaac']
　　['1201','gaacaattct','acagtccaga','atgtaggagg','ggcttatgaa','tttcatgctg','gagatgtaat']
　　['1261','agacttgata','ttcaatgctg','aacatcaggt','tctaaaaaat','aatacatact','gggggatctt']
　　['1321','tctgctagca','aatccccaat','tcatctccta','gagactcagt','taggtctcct','catcttcagc']
　　['1381','acatgcagag','atgccagtgc','ataggatgga','gaaggaagat','tttcaacaca','tacagttcat']
　　['1441','ctgggtatac','aaatcaacat','gaacagatct','cctctgcatg','tgaagcttca','tttctcctgc']
　　['1501','ttattgaatg','agactcagaa','agcactgaag','acatttggtt','acccctgatg','ttgggtcagc']
　　['1561','aaagacactt','tactagttca','tgataaaatg','aaaatgggtg','gctggaagac','aaaatctttt']
　　['1621','caaagtgtct','gtctaatcct','tgaacccctg','agtggaaaaa','tgaggtctat','tcccataata']
　　['1681','gccttatata','gcatgcaaaa','aaagaccagg','gcagtagcct','ggtcttgttc','ttatattctt']
　　['1741','ggactgtgga','ctgtttcaat','tcattcttcc','catattctca','tcttaggaga','cactcttaat']
　　['1801','aaaatgtagt','cagagtgggt','gtgtggccag','caacactcca','ttttggagtt','gatgagatta']
　　['1861','ggggatagag','aacactctta','ggaaatattg','ggacagaatt','tcagttggca','ttgaaatgga']
　　['1921','atgcacttta','ttcgggaatt','tcacttgatt','tcatcatcaa','gtgcagggtg','ctctataaaa']
　　['1981','cctgctggtc','aaaaggctag','ctttcaatct','tcacatagca','gttcatgaga','atttactggt']
　　['2041','gtatgtatct','aaccatgtca','atgacaaaga','gtaatcatta','gtagtaagat','ctaacccccc']
　　['2101','aaattggtat','taccagtact','gtactttgca','actgtgcaga','gccagctaaa','aatatgaaat']
　　['2161','cattacatga','caaagcactt','tcatatacca','catggcaact','cgatagattt','aatggggcag']
　　['2221','atatttttgg','cacaatttta','actatgagaa','tacagaggca','gatggaatag','aggtaacttg']
　　['2281','gttcagttca','tagaactagt','aattaacagg','cacctgggct','tcccactgta','ttatactata']
　　['2341','ttagctttac','gattggtatt','tctgctatca','tgttagaagc','ctataaactt','taacagattt']
　　['2401','aaaattttca','gacagtatat','tcccttttag','tccaacagca','attttttcct','ttctcagcaa']
　　['2461','atttcttttc','ttttctttgc','ctggagcagg','gtacccaggg','tgttattcaa','gacttactac']
　　['2521','aacttaatct','ccttccttac','tttggtcaaa','tgtgttaact','tccaaaaata','atgaataata']
　　['2581','ctcaattcag','ggacagtctg','ttaaattttt','ggactctgca','aaattaacta','gctgcttatg']
　　['2641','ggttgttatt','aaaaggtatg','taggtaatgt','gattacatga','aaacccaatt','taaaatattt']
　　['2701','atggatattt','gtaaaaaatc','tacattatgt','taattaatag','tatcaccatt','aaaaactaat']
　　['2761','ttaagaatat','ttgtattgta','tgtaagaaaa','actgcttgga','agcagactaa','gcctgaggcc']
　　['2821','aagatgcctc','atagtatgtc','tttttttttt','tttttttaaa','tacatctgct','gagcagctgt']
　　['2881','agggacaaag','actggggtac','ctggttcctc','ttgtatttgt','gtatcatctc','aggaaattaa']
　　['2941','agttacataa','catacatata','tttatggaaa','cgtggtattg','atgttaactt','ataagcagta']
　　['3001','gtgtgctgga','gtgggctagc','actagctcag','gagagctgtt','aaatttttat','taattgtgta']
　　['3061','gtctggttat','taaatcatta','tccttgaaat','tggccatggt','aggacaattt','ataccatgtg']
　　['3121','aattagcaaa','tgctacaaat','cagggctttt','ctttttggaa','agcccatgca','ccagcacacc']
　　['3181','actgtttata','aaactcttct','taatgactcc','tctcagcccc','tgcctcagta','ttacaacagt']
　　['3241','caaggcaggc','aaggaaagtg','tcttactctc','agcaaaagcc','ccacagataa','atcatttctc']
　　['3301','agggcaggtg','gaggaatcta','cagctgtaac','cagatagata','gctaccaaca','tatgaccttt']
　　['3361','gaatttccct','agtgttgaaa','tttcaggctt','tgttttcaat','gtatactctg','ttcccttgtt']
　　['3421','tcttcaaaac','agtgtttata','ttttaaactg','acaataaaat','gtttgtacat','gggctgtagc']
　　['3481','tgatttatct','atgggttatc&